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090			_aB-10012
245	1	0	_aGlobal identification of microRNA-target RNA pairs by parallel analysis of RNA ends
490	0		_vNature Biotechnology, 26(8), p.941-946, 2008
520	3		_aMicroRNAs (miRNAs)are important regulatory molecules in most eukaryotes and identification of their target mRNAs is essential for their functional analysis. Whereas conventional methods rely on computational prediction and subsequent experimental validation of target RNAs, we directly sequenced >28,000,000 signatures from the 5_O ends of polyadenylated products of miRNA-mediated mRNA decay, isolated from inflorescence tissue of Arabidopsis thaliana, to discover novel miRNA.target RNA pairs. Within the set of ~27,000 transcripts included in the 8,000,000 nonredundant signatures, several previously predicted but nonvalidated targets of miRNAs were found. Like validated targets, most showed a single abundant signature at the miRNA cleavage site, particularly in libraries from a mutant deficient in the 5_O-to-3_O exonuclease AtXRN4. Although miRNAs in Arabidopsis have been extensively investigated, working in reverse from the cleaved targets resulted in the identification and validation of novel miRNAs. This versatile approach will affect the study of other aspects of RNA processing beyond miRNA.target RNA pairs.
700	1	2	_aGerman, M.A.
700	1	2	_aPillay, M.
700	1	2	_aJeong, D.
700	1	2	_aHetawal, A.
700	1	2	_aLuo, S.
700	1	2	_aJanardhanan, P.
700	1	2	_aKannan, V.
700	1	2	_aKannan, V.
700	1	2	_aNobuta, K.
700	1	2	_aGerman, R.
700	1	2	_aDe Paoli, E.
700	1	2	_aLu, C.
700	1	2	_aSchroth, G.
700	1	2	_aMeyers, B.C.
700	1	2	_aGreen, P.J.
856	4	0	_uhttps://drive.google.com/file/d/1mihwvVvEdoO24eASeuTePwT-IDihPHWk/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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