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245	1	0	_aTwo-polymerase mechanisms dictate error-free and error-prone translesion DNA synthesis in mammals
490	0		_vThe EMBO Journal, 28, p.383-393, 2008
520	3		_aDNA replication across blocking lesions occurs by translesion DNA synthesis (TLS), involving a multitude of mutagenic DNA polymerases that operate to protect the mammalian genome. Using a quantitative TLS assay, we identified three main classes of TLS in human cells: two rapid and error-free, and the third slow and error-prone. A single gene, REV3L, encoding the catalytic subunit of DNA polymerase f (polf), was found to have a pivotal role in TLS, being involved in TLS across all lesions examined, except for a TT cyclobutane dimer. Genetic epistasis siRNA analysis indicated that discrete two-polymerase combinations with polf dictate error-prone or error-free TLS across the same lesion. These results highlight the central role of polf in both error-prone and error-free TLS in mammalian cells, and show that bypass of a single lesion may involve at least three different DNA polymerases, operating in different two-polymerase combinations.
700	1	2	_aShachar, S.
700	1	2	_aZiv, O.
700	1	2	_aAvkin, S.
700	1	2	_aAdar, S.
700	1	2	_aWittschieben, J.
700	1	2	_aReißner, T.
700	1	2	_aChaney, S.
700	1	2	_aChaney, S.
700	1	2	_aWang, Z.
700	1	2	_aCarell, T.
700	1	2	_aGeacintov, N.
700	1	2	_aLivneh, Z.
856	4	0	_uhttps://drive.google.com/file/d/1Wqf0FvzoMe0XzMNY2ptdW7qTgUVU-8A2/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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