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| 003 | MX-MdCICY | ||
| 005 | 20250625140630.0 | ||
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| 090 | _aB-10373 | ||
| 245 | 1 | 0 | _aSecretome analysis of differentially induced proteins in rice suspension-cultured cells triggered by rice blast fungus and elicitor |
| 490 | 0 | _vProteomics, 9(5), p.1302-1313, 2009 | |
| 520 | 3 | _aSecreted proteins were investigated in rice suspension-cultured cells treated with rice blast fungus Magnaporthe grisea and its elicitor using biochemical and 2-DE coupled with MSanalyses followed by their in planta mRNA expression analysis. M. grisea and elicitor successfully interacted with suspension-cultured cells and prepared secreted proteins from these cultures were essentially intracellular proteins free. Comparative 2-D gel analyses identified 21 differential protein spots due to M. grisea and/or elicitor over control. MALDI-TOF-MS and mLC-ESI-MS/MS analyses of these protein spots revealed that most of assigned proteins were involved in defense such as nine chitinases, two germin A/oxalate oxidases, five domain unknown function 26 (DUF 26)secretory proteins, and b-expansin. One chitin binding chitinase protein was isolated using chitin binding beads and strong enzymatic activity was identified in an in-gel assay. Interestingly, their protein abundance correlated well at transcript levels in elicitor-treated cultures as judged by semi-quantitative RT-PCR. Each identified differentially expressed protein group was compared at transcript levels in rice leaves inoculated with incompatible (KJ401)and compatible (KJ301)races of M. grisea. Time-course profiling revealed their inductions were stronger and earlier in incompatible than compatible interactions. Identified secreted proteins and their expression correlation at transcript level in suspension-cultured cells and also in planta suggest that suspension-cultured cells can be useful to investigate the secretome of rice blast-pathogen interactions. | |
| 650 | 1 | 4 | _aELICITOR |
| 650 | 1 | 4 | _aMALDI-TOF-MS |
| 650 | 1 | 4 | _aM. GRISEA |
| 650 | 1 | 4 | _aSECRETOME |
| 650 | 1 | 4 | _aSUSPENSION-CULTURED CELL |
| 700 | 1 | 2 | _aKim, S.T. |
| 700 | 1 | 2 | _aKang, Y.H. |
| 700 | 1 | 2 | _aWang, Y. |
| 700 | 1 | 2 | _aWu, J. |
| 700 | 1 | 2 | _aPark, Z.Y. |
| 700 | 1 | 2 | _aRakwal, R. |
| 700 | 1 | 2 | _aAgrawal, G.K. |
| 700 | 1 | 2 | _aAgrawal, G.K. |
| 700 | 1 | 2 | _aKang, K.Y. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/14edaSBmB-Ww6pGgqwzAK_g_chvXDRAdw/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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