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090			_aB-10457
245	1	0	_aRecombination and expression of classical swine fever virus (CSFV)structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts
490	0		_vColloids and Surfaces B: Biointerfaces, 55(1), p.26-30, 2007
520	3		_aThe expression of classical swine fever virus (CSFV)structural protein E2 in different vectors, which has been shown to carry critical epitopes, has been established. Here, we reported a Chlamydomonas reinhardtii chloroplast expression vector, P64E2, containing classical swine fever virus structural protein E2 gene, which was constructed and transferred to C. reinhardtii by biolistic bombardment method. The transformants were identified by PCR, Southern blotting, Western blotting after selecting on resistant media. ELISA quantification assay showed that the expressed E2 protein accumulated up to 1.5-2
650	1	4	_aCLASSICAL SWINE FEVER VIRUS (CSFV)
650	1	4	_aE2 GENE
650	1	4	_aCHLAMYDOMONAS REINHARDTII
650	1	4	_aCHLOROPLAST TRANSFORMATION
650	1	4	_aANTI-DISEASE PROTEIN
650	1	4	_aBIOINTERFACE
650	1	4	_aPLANT GENE ENGINEERING
700	1	2	_aHe, D.M.
700	1	2	_aQian, K.X.
700	1	2	_aShen, G.F.
700	1	2	_aZhang, Z.F.
700	1	2	_aLie, Y.N.
700	1	2	_aSud, Z.L.
700	1	2	_aShao, H.B.
856	4	0	_uhttps://drive.google.com/file/d/1jDsK7jMNxQoyc7fEkp-oPz0uhHnVpzvX/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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