| 000 | 02649nam a2200277Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625140632.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-10493 | ||
| 245 | 1 | 0 | _aThe characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage in costal cartilage defects |
| 490 | 0 | _vActa Biomaterialia, 136, p.147-158, 2021 | |
| 520 | 3 | _aAfter harvesting multiple costal cartilages, the local defect disrupts the integrity of the chest wall and may lead to obvious thoracic complications, such as local depression and asymmetry of the bilateral thoracic height. Decellularized materials have been used for tissue reconstruction in clinical surgeries. To apply xenogenic decellularized cartilage in costal cartilage defects, porcine-derived auricular and costal cartilage was tested for characterization, cytotoxicity, macrophage response, and tissue regeneration. Most of the DNA and a-Gal were effectively removed, and the collagen was well preserved after the decellularization process. The glycosaminoglycan (GAG)content decreased significantly compared to that in untreated cartilage. The decellularized auricular cartilage had a larger pore size, more pores, and a higher degradation rate than the decellularized costal cartilage. No apparent nuclei or structural damage was observed in the extracellular matrix. The decellularized auricular cartilage had a higher cell proliferation rate and more prominent immunomodulatory effect than the other groups. Two types of decellularized cartilage, particularly decellularized auricular cartilage, promoted the tissue regeneration in the cartilage defect area, combined with noticeable cartilage morphology and increased chondrogenic gene expression. In our research, the functional components and structure of the extracellular matrix were well preserved after the decellularization process. The decellularized cartilage had better biocompatibility and suitable microenvironment for tissue regeneration in the defect area, suggesting its potential application in cartilage repair during the surgery. | |
| 650 | 1 | 4 | _aCOSTAL CARTILAGE DEFECT |
| 650 | 1 | 4 | _aDECELLULARIZED CARTILAGE |
| 650 | 1 | 4 | _aTHORACIC DEFORMITY |
| 650 | 1 | 4 | _aMACROPHAGE RESPONSE |
| 700 | 1 | 2 | _aChangchen, W. |
| 700 | 1 | 2 | _aHongquan, W. |
| 700 | 1 | 2 | _aBo, Z., |
| 700 | 1 | 2 | _aLeilei, X., |
| 700 | 1 | 2 | _aHaiyue, J. |
| 700 | 1 | 2 | _aBo, P. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/17Cwl5AzddOyw9ZfvBsRNBMAbIKJNWpmB/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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