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| 003 | MX-MdCICY | ||
| 005 | 20250625140633.0 | ||
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| 090 | _aB-10506 | ||
| 245 | 1 | 0 | _aThe Yeast Signal Sequence Trap Identifies Secreted Proteins of the Hemibiotrophic Corn Pathogen Colletotrichum graminicola |
| 490 | 0 | _vMolecular Plant-Microbe Interactions, 21(10), p.1325-1336, 2008 | |
| 520 | 3 | _aThe hemibiotroph Colletotrichum graminicola is the causal agent of stem rot and leaf anthracnose on Zea mays. Following penetration of epidermal cells, the fungus enters a short biotrophic phase, followed by a destructive necrotrophic phase of pathogenesis. During both phases, secreted fungal proteins are supposed to determine progress and success of the infection. To identify genes encoding such proteins, we constructed a yeast signal sequence trap (YSST)cDNA-library from RNA extracted from mycelium grown in vitro on corn cell walls and leaf extract. Of the 103 identified unigenes, 50 showed significant similarities to genes with a reported function, 25 sequences were similar to genes without a known function, and 28 sequences showed no similarity to entries in the databases. Macroarray hybridization and quantitative reverse-transcriptase polymerase chain reaction confirmed that most genes identified by the YSST screen are expressed in planta. Other than some genes that were constantly expressed, a larger set showed peaks of transcript abundances at specific phases of pathogenesis. Another set exhibited biphasic expression with peaks at the biotrophic and necrotrophic phase. Transcript analyses of in vitro-grown cultures revealed that several of the genes identified by the YSST screen were induced by the addition of corn leaf components, indicating that host-derived factors may have mimicked the host milieu. | |
| 650 | 1 | 4 | _aBIOTROPHY |
| 650 | 1 | 4 | _aCELL-WALL-DEGRADING ENZYMES |
| 650 | 1 | 4 | _aEFFECTOR PROTEINS |
| 650 | 1 | 4 | _aNECROTROPHY |
| 650 | 1 | 4 | _aPEPTIDASES |
| 700 | 1 | 2 | _aKrijger, J.J. |
| 700 | 1 | 2 | _aHorbach, R. |
| 700 | 1 | 2 | _aBehr, M. |
| 700 | 1 | 2 | _aSchweizer, P. |
| 700 | 1 | 2 | _aDeising, H.B. |
| 700 | 1 | 2 | _aWirsel, S.G.R. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1vOJopYinbvvG-k26bMdLjc9seIdDSI2R/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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