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040			_cCICY
090			_aB-10567
245	1	0	_aTransposon-tagging identifies novel pathogenicity genes in Fusarium graminearum
490	0		_vFungal Genetics and Biology, 45(12), p.1552-1561, 2008
520	3		_aWith the increase of sequenced fungal genomes, high-throughput methods for functional analyses of genes are needed. We assessed the potential of a new transposon mutagenesis tool deploying a Fusarium oxysporum miniature inverted-repeat transposable element mimp1, mobilized by the transposase of impala, a Tc1-like transposon, to obtain knock-out mutants in Fusarium graminearum. We localized 91 mimp1 insertions which showed good distribution over the entire genome. The main exception was a major hotspot on chromosome 2 where independent insertions occurred at exactly the same nucleotide position. Furthermore insertions in promoter regions were over-represented. Screening 331 mutants for sexual development, radial growth and pathogenicity on wheat resulted in 19 mutants (5.7
650	1	4	_aTRANSPOSON MUTAGENESIS
650	1	4	_aFUSARIUM GRAMINEARUM
650	1	4	_aFUNCTIONAL SCREENING
650	1	4	_aPATHOGENICITY
650	1	4	_aSEXUAL DEVELOPMENT
700	1	2	_aDufresne, M.
700	1	2	_aVan Der Lee, T.
700	1	2	_aM'Barek, S.B.
700	1	2	_aXu, X.
700	1	2	_aZhang, X.
700	1	2	_aLiu, T.
700	1	2	_aWaalwijk, C.
700	1	2	_aWaalwijk, C.
700	1	2	_aKema, G.H.J.
700	1	2	_aDaboussi, M.J.
856	4	0	_uhttps://drive.google.com/file/d/1MSS-w76mEjoj3obUnV2bjNAbd50Kg1bL/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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