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090			_aB-10669
245	1	0	_aComparative Proteomic Analysis of Botrytis cinerea Secretome
490	0		_vJournal of Proteome Research, 8(3), p.1123-1130, 2009
520	3		_aBotrytis cinerea (B. cinerea)is a filamentous fungus infecting more than 200 plant species, causing significant economic losses worldwide. Secreted proteins are released as an initial response of the fungus to its plant host. We report the use of a high-throughput LC-MS/MS approach to analyze B. cinerea BO5.10 secreted proteins. Secretions were collected from fungus grown on a solid substrate of cellophane membrane while mock infecting media supplemented with the extract of full red tomato, ripened strawberry or Arabidopsis leaf extract. Overall, 89 B. cinerea proteins were identified from all growth conditions. Sixty proteins were predicted to contain a SignalP motif indicating the extracellular location of the proteins. Seven proteins were observed in all the growth conditions implying a constitutive nature of their secretion. Identified in the secretions were transport proteins, proteins wellcharacterized for carbohydrate metabolism, peptidases, oxidation/reduction, and pathogenicity factors that provide important insights into how B. cinerea may use secreted proteins for plant infection and colonization.
650	1	4	_aB. CINEREA
650	1	4	_aSECRETED PROTEINS
650	1	4	_aCERATOPLATANIN
650	1	4	_aMASS SPECTROMETRY
700	1	2	_aShah, P.
700	1	2	_aAtwood, J.A.
700	1	2	_aOrlando, R.
700	1	2	_aMubarek, H.E.
700	1	2	_aPodila, G.P.
700	1	2	_aDavis, M.R.
856	4	0	_uhttps://drive.google.com/file/d/1LtKeCFajd1FYzokMjmn7QCEDPgV-THDm/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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