| 000 | 01380nam a2200241Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625140638.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-10776 | ||
| 245 | 1 | 0 | _aA silver staining procedure for nucleic acids in polyacrylamide gels without fixation and pretreatment |
| 490 | 0 | _vAnalytical BioChemistry, 391(1), p.77-79, 2009 | |
| 520 | 3 | _aSilver staining of nucleic acid has been widely used in molecular marker analysis such as simple sequence repeat (SSR), single-strand conformation polymorphism (SSCP), and amplified fragment-length polymorphism (AFLP). Many alternatives to silver staining methods have been described, but these methods are not efficient or cost-effective. Here we report a silver staining method that requires less than 10 min for one gel and can save chemicals as well. It has a detection limit of approximately 5.6 pg of DNA/mm2 in nondenaturing polyacrylamide gels and 12.8 pg/mm2 in denaturing polyacrylamide gels. | |
| 700 | 1 | 2 | _aAn, Z.W. |
| 700 | 1 | 2 | _aXie, L.L. |
| 700 | 1 | 2 | _aCheng, H. |
| 700 | 1 | 2 | _aZhou, Y. |
| 700 | 1 | 2 | _aZhang, Q. |
| 700 | 1 | 2 | _aHe, X.G. |
| 700 | 1 | 2 | _aHuang, H.S. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1ikCCnAkDuaZ9BTzSkWlHJurbYiowYmdd/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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