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090 _aB-10846
245 1 0 _aAn integrated genetic linkage map of avocado
490 0 _vTheoretical and Applied Genetics, 95(5-6), p.911-921, 1997
520 3 _aAn avocado genomic library was screened with various microsatellite repeats. (A/T)/ and (TC/AG)/ sequences were found to be the most frequent repeats. One hundred and seventy-two positive clones were sequenced successfully of which 113 were found to contain simple sequence repeats (SSR). Polymerase chain reaction primers were designed to the regions ßanking the SSR in 62 clones. A GenBank search of avocado DNA sequences revealed 1 sequence containing a (CT)10 repeat. A total of 92 avocadospeci Þc SSR markers were screened for polymorphism using 50 o¤spring of a cross between the avocado cultivars ÔPinkertonÕ and ÔEttingerÕ. Both are standard avocado cultivars which are normally outcrossed and highly heterozygous. Fifty polymorphic SSR loci, 17 random ampliÞed polymorphic DNA (RAPD)and 23 minisatellite DNA Fingerprint (DFP)bands were used to construct the avocado genetic map. The resulting data were analyzed with various mapping programs in order to assess which program best accommodated data from progeny of heterozygous parents. The analyses resulted in 12 linkage groups with 34 markers (25 SSRs, 3 RAPDs and 6 DFP bands)covering 352.6 cM. This initial map can serve as a basis for developing a detailed genomic map and for detection of linkage between markers and quantitative trait loci.
650 1 4 _aLINKAGE GROUPS
650 1 4 _aMENDELIAN INHERITANCE
650 1 4 _aMICROSATELLITES
650 1 4 _aMINISATELLITES
650 1 4 _aSIMPLE SEQUENCE REPEATS
650 1 4 _aVARIABLE NUMBER OF TANDEM REPEATS
700 1 2 _aSharon, D.
700 1 2 _aCregan, P.B.
700 1 2 _aMhameed, S.
700 1 2 _aKusharska, M.
700 1 2 _aHillel, J.
700 1 2 _aLahav, E.
700 1 2 _aLavi, U.
856 4 0 _uhttps://drive.google.com/file/d/1N9VFVTqj4qsvykf7pLQiR9uTaF81PlB6/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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