000			02279nam a2200253Ia 4500
003			MX-MdCICY
005			20250625140639.0
040			_cCICY
090			_aB-10847
245	1	0	_aAntigenic relationships between petunia peroxidase a and specific peroxidase isoenzymes in other Solanaceae
490	0		_vTheoretical and Applied Genetics, 80(1), p.113-120, 1990
520	3		_aA highly specific rabbit antiserum raised against peroxidase (PRXa)from petunia (Petunia hybrida)was used to investigate the antigenic relatedness of peroxidases in the Solanaceae. After SDS-PAGE of crude leaf extracts from a large number of species of this family, immunoblotting revealed that cross-reacting protein bands were present in all species tested. In order to determine whether these protein bands represent peroxidases, the peroxidase isoenzymes in thorn apple (Datura stramonium L.), tobacco (Nicotiana tabacum L.), sweet pepper (Capsicum annuum L.), potato (Solanum tuberosum L.), and tomato (Lycopersicon esculentum Mill.)were further analyzed. Immunoblots obtained after native PAGE revealed that the antiserum only recognized fast-moving peroxidase isoenzymes that are localized in the apoplast. Despite their serological relatedness, these peroxidases differed with respect to heat stability and apparent molecular weight. Differences in avidity for the petunia PRXa antiserum were suggested by immunoprecipitation with antibodies bound to protein A-Sepharose. The antiserum did not react with peroxidases from horseradish (Armoracea rusticana Gaertn., Mey and Scherb), turnip (Brassica napus L.), African marigold (Tagetes cresta L.), maize (Zea mays L.), and oats (Arena sativa L.). Apparently, the Solanaceae contain orthologous genes encoding the fast-moving anionic peroxidases homologous to petunia PRXa.
650	1	4	_aIMMUNOLOGY
650	1	4	_aPEROXIDASE ISOENZYMES
650	1	4	_aPETUNIA
650	1	4	_aSOLANACEAE
700	1	2	_aHendriks, T.
700	1	2	_aDe Jong, A.
700	1	2	_aWijsman, H. J. W.
700	1	2	_aVan Loon, L.C.
856	4	0	_uhttps://drive.google.com/file/d/1030bu3r_TN3deQsA3mITvPpe4RRy40X1/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c45078 _d45078