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090			_aB-11068
245	1	0	_aCharacterisation and changes in the antioxidant system of chloroplasts and chromoplasts isolated from green and mature pepper fruits
490	0		_vPlant Biology, 11(4), p.613-624, 2009
520	3		_aPurification and characterisation of pepper (Capsicum annuum L)chloroplasts and chromoplasts isolated from commercial green, red and yellow mature fruits were undertaken. Induction of the synthesis of several antioxidants in organelles isolated from mature fruits was found. The ultrastructure of organelles and the presence and activity of SOD isozymes and enzymes involved in the ASC-GSH cycle, together with the non-enzymatic antioxidant content and some oxidative parameters, were analysed. It was found that lipids, rather than proteins, seem to be a target for oxidation in the chromoplasts. The ascorbate and glutathione contents were elicited during differentiation of chloroplasts into chromoplasts in both red and yellow fruits. The activity of SOD and of components of the ASC-GSH cycle was up-regulated, suggesting that these enzymes may play a role in the protection of plastids and could act as modulators of signal molecules such as O2 ·)and H2O2 during fruit maturation. The presence of an Mn-SOD in chromoplasts isolated from yellow pepper fruits was also investigated in terms of structural and antioxidant differences between the two cultivars.
650	1	4	_aASCORBATE
650	1	4	_aGLUTATHIONE
650	1	4	_aOXIDATION
650	1	4	_aPLASTID
650	1	4	_aRIPENING
650	1	4	_aSOD
700	1	2	_aMartí, M.C.
700	1	2	_aCamejo, D.
700	1	2	_aOlmos, E.
700	1	2	_aSandalio, L.M.
700	1	2	_aFernández-García, N.
700	1	2	_aJiménez, A.
700	1	2	_aSevilla, F.
856	4	0	_uhttps://drive.google.com/file/d/1i2q6aUY0F6Dv9-kRyYrUVTfr-wSYT7TJ/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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