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090			_aB-11079
245	1	0	_aAssessing the limitations to terpenoid indole alkaloid biosynthesis in Catharanthus roseus hairy root cultures through gene expression profiling and precursor feeding
490	0		_vBiotechnology Progress, 25(5), p.1289-1296, 2009
520	3		_aThe production of pharmaceutically important terpenoid indole alkaloids (TIAs)from Catharanthus roseus is partly regulated at the transcriptional level. In this study, limitations in TIA biosynthesis from C. roseus hairy root cultures were assessed through gene expression profiling and precursor feeding. The transcript levels of key TIA pathway genes (G10h, Tdc, Str, and Sgd)and metabolite levels associated with the TIA pathway (tryptamine, loganin, secologanin, strictosidine, ajmalicine, serpentine, and tabersonine)were monitored using quantitative RT-PCR and HPLC, respectively. In cultures elicited with methyl jasmonate (250 M MeJA on day 21), G10h, Tdc, Str, and Sgd expression increased by 9.1, 3.1, 6.7, and 8.3-fold, respectively, after 24 h. Up-regulation of gene expression was followed by a 160, 440, and 420
650	1	4	_aCATHARANTHUS ROSEUS
650	1	4	_aHAIRY ROOT CULTURES
650	1	4	_aGENE EXPRESSION
650	1	4	_aMETHYL JASMONATE
650	1	4	_aQUANTITATIVE RT-PCR
650	1	4	_aALKALOID
650	1	4	_aPRECURSOR FEEDING
700	1	2	_aGoklany, S.
700	1	2	_aLoring, R.H.
700	1	2	_aGlick, J.
700	1	2	_aLee-Parsons, C.W.T.
856	4	0	_uhttps://drive.google.com/file/d/1iAgGeCnQo8MtN5lMJWqxK6JZ2KhAxP41/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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