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090			_aB-11141
245	1	0	_aImmobilization and characterization of porcine pancreas lipase
490	0		_vEnzyme and Microbial Technology, 20(7), p.351-535, 1997
520	3		_aPorcine pancreas lipase (triacylglycerol ester hydrolase, EC 3.1.1.3)was immobilized with the highest activity (2,187 U g-t solid)on polyactylamide beads possessing carboxylic functional groups activated by a watersoluble carbodiimide. The optimum pH for catalytic activity was pH 8.9. The apparent optimum temperature for the immobilized enzyme was about 7°C higher than that for the soluble enzyme. The immobilization stabilized the enzyme against heat and urea treatment. Cross-linking of the immobilized enzyme with glutaraldehyde or 3,5-difluoronitrobenzene improved the thermal stability. Application of the immobilized lipase for olive oil hydrolysis is also presented.
650	1	4	_aPORCINE PANCREAS LIPASE
650	1	4	_aIMMOBILIZATION
650	1	4	_aCROSS-LINKING
650	1	4	_aCONFORMATIONAL STABILITY
700	1	2	_aBagi, K.
700	1	2	_aSimon, L. M.
700	1	2	_aSzajani, B.
856	4	0	_uhttps://drive.google.com/file/d/1b6lUCJuadTMhSnkSnISy6O81QREMrik7/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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