| 000 | 01534nam a2200241Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625140645.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-11184 | ||
| 245 | 1 | 0 | _aThe use of the nonradioactive digoxigenin chemiluminescent technology for plant genomic Southern blot hybridization: a comparison with radioactivity |
| 490 | 0 | _vTransgenic Research, 2(2), p.115-120, 1993 | |
| 520 | 3 | _aA nonradioactive labelling and detection method for plant genomic DNA analysis is compared to the radioactive method. The radioisotopes are replaced by a nucleotide, digoxigenin-11-dUTP, and the signal detection is accomplished by the enzymatic reaction of alkaline phosphatase, conjugated to anti-digoxigenin antibodies, with the chemiluminescent substrate AMPPD 3-(2'-spiroadamantane)-4-methoxy-4(3"phosphorytoxy)phenyl-1, 2-dioxetane). The sensitivity of the radioactive and nonradioactive methods are directly compared using identical Southern blots subjected to the radioactive and nonradioactive detection. The advantages of this nonradioactive method are discussed. | |
| 650 | 1 | 4 | _aCHEMILUMINESCENCE |
| 650 | 1 | 4 | _aDIGOXIGENIN |
| 650 | 1 | 4 | _aNONRADIOACTIVE |
| 650 | 1 | 4 | _aPLANT DNA |
| 650 | 1 | 4 | _aSOUTHERN BLOT HYBRIDIZATION |
| 700 | 1 | 2 | _aNeuhaus-Url, G. |
| 700 | 1 | 2 | _aNeuhaus, G. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/17vGVLjlNja-QFc3bes7AbbFjy6UJzg6y/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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