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245 1 0 _aInfluence of 8-Methyl-nonenoic Acid on Capsaicin Biosynthesis in In-Vivo and In-Vitro Cell Cultures of Capsicum Spp.
490 0 _vJ. Agric. Food Chem., 54(5), p.1854-1859, 2006
520 3 _aCapsaicin is a bioactive molecule synthesized by enzymatic (putative capsaicin synthase)condensation of vanillylamine, a phenyl propanoid intermediate with 8-methyl-nonenoic acid, a fatty acid derivative from leucine/valine pathway. Analysis of levels of 8-methyl-nonenoic acid and phenyl propanoid intermediates in high, medium, and low pungent Capsicum genotypes revealed that the 8-methylnonenoic acid pool plays a crucial role in determining the efficacy of capsaicin levels. Ceruleninmediated inhibition of 8-methyl-nonenoic acid synthesis decreased the capsaicin biosynthesis in Capsicum cell suspension cultures. Similarly amino oxy acetate inhibited vanillylamine synthesis but failed to reduce capsaicin production. The mRNA transcript analysis of keto acyl synthase (KAS), a crucial enzyme involved in 8-methyl-nonenoic acid and an amino transferase (AMT), involved in vanillylamine biosynthesis was studied. The mRNA transcript analysis revealed the progressive developmental expression of KAS gene in the placenta during the ontogeny of the fruit, whereas AMT transcripts levels did not show significant differences. Hence, the study demonstrates the influence of 8-methyl-nonenoic acid and its possible regulatory role in capsaicin biosynthesis in Capsicum spp.
650 1 4 _aCAPSICUM SPP.
650 1 4 _aCAPSAICIN SYNTHASE
650 1 4 _aVANILLYLAMINE
650 1 4 _a8-METHYL-NONENOIC ACID
650 1 4 _aMRNA TRANSCRIPTS
650 1 4 _aCERULENIN
650 1 4 _aAMINO OXYACETATE
700 1 2 _aBellur Chayapathy Narasimha Prasad
700 1 2 _aHarishchandra Bhaskar Gururaj
700 1 2 _aVinod Kumar, Parvatam Giridhar
700 1 2 _aRangan Parimalan
700 1 2 _aAshwani Sharma
700 1 2 _aGokare Ashwathnarayana Ravishankar
856 4 0 _uhttps://drive.google.com/file/d/1scKIX3RWBjRczTjPdxPHUjX57xQ736DH/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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