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245	1	0	_aCerium elicitor-induced phosphatidic acid triggers apoptotic signaling development in Taxus cuspidata cell suspension cultures
490	0		_vChemistry and Physics of Lipids, 159(1), p.13-20, 2009
520	3		_aDegradation of membrane phospholipids is associated with apoptotic responses, but the signaling development of this degradation is not well understood. Cerium (Ce4+), an important rare earth element, induces cellular apoptosis and taxol biosynthesis in Taxus cuspidata suspension cultures. Here, using mass spectrometry and biochemical technique, we demonstrated that the phospholipase D (PLD)was rapidly activated by Ce4+ and hydrolyzed structural phospholipids to generate lipid signal molecule, phosphatidic acid (PA). 1-Butanol, an antagonist of PLD-dependent PA production, blocked the biphasic burst of superoxide anions (O2 -)and thus mitigated cellular apoptosis. The time-course analysis of PA accumulation and ERK-like mitogen-activated protein kinase (MAPK)regulation indicated PA generation preceded MAPK activation, suggesting that the rapid accumulation of PA might be required for the initial MAPK activity. After 2 h of Ce4+ elicitation, however, PA-induced O2 - burst, forming a negative regulation to MAPK activity, which in turn led to apoptotic signaling development.
650	1	4	_aPHOSPHATIDIC ACID
650	1	4	_aPHOSPHAPLIPASE D
650	1	4	_aLC-MS
650	1	4	_aAPOPTOSIS
650	1	4	_aSIGNALING DEVELOPMENT
650	1	4	_aTAXUS CUSPIDATA CELLS
700	1	2	_aYang, S.
700	1	2	_aLu, S.
700	1	2	_aYuan, Y.
856	4	0	_uhttps://drive.google.com/file/d/1r1QBhETCcm-9mUlw1aCjFYxdHZJ3QSPX/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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