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003			MX-MdCICY
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040			_cCICY
090			_aB-11331
245	1	0	_aRNA complementary to the 50 UTR of mRNA triggers effective silencing in Saccharomyces cerevisiae
490	0		_vBioChemical and Biophysical Research Communications, 339(4), p.1224-1231, 2006
520	3		_aConditional silencing of target genes in Saccharomyces cerevisiae by antisense RNAs expressed in vivo has been challenged. The MFa1::lacZ fusion present in S. cerevisiae SF51-3 was chosen as a model target, and fragments of this gene were cloned in reverse orientation into the expression vector pYES2, bearing the GAL1 promoter. Among the different antisense constructs tested, only the one complementary to the 50 UTR of target mRNA featured effective silencing. Nevertheless, the expression in vivo of this antisense RNA could not be properly tuned by the absence or presence of galactose in the culture medium. Accordingly, conditional silencing could not be attained by this antisense hosted into pYES2. On the contrary, cloning the same antisense construct into the expression vector pSAL4 yielded a fully conditional silencing linked to the control of antisense expression by the absence or presence of Cu2+ into the culture medium.
650	1	4	_aSACCHAROMYCES CEREVISIAE
650	1	4	_aYEAST
650	1	4	_aANTISENSE
650	1	4	_aRNA
650	1	4	_aSILENCING
650	1	4	_a50 UTR
650	1	4	_aPYES2
650	1	4	_aPSAL4
700	1	2	_aBonoli, M.
700	1	2	_aGraziola, M.
700	1	2	_aPoggi, V.
700	1	2	_aHochkoeppler, A.
856	4	0	_uhttps://drive.google.com/file/d/14rRd63KmK99fgpZeZaHR7MZw7BrBnVZh/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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