000			02101nam a2200289Ia 4500
003			MX-MdCICY
005			20250625140649.0
040			_cCICY
090			_aB-11409
245	1	0	_aA simple test using restricted PCR-amplifled mitochondrial DNA to study the genetic structure of Apis mellifera L.
490	0		_vCelular and Molecular Life Science, 49(1), p.1016-1021, 1993
520	3		_aThe COI-COII intergenic region of Apis mellifera mitochondrial DNA contains an important length polymorphism based on a variable number of copies of a 192 196 bp sequence (Q)and the complete or partial deletion of 67 bp sequence (Po). This length variability has been combined with a restriction site polymorphism to produce a rapid and simple test for the characterization of mtDNA haplotypes. This test includes the amplification by the polymerase chain reaction of the COI-COII region followed by a DraI restriction of the amplified fragment. In a survey of 302 colonies belonging to 12 subspecies, 21 different haplotypes have been found which have been unambiguously allocated to one of the 3 mtDNA lineages of the species. Although all colonies of lineage C exhibit the same pattern (C1), each one of lineages A and M presents up to 10 different haplotypes, opening the way to studies on the genetic structure and the evolution of a large fraction of the species. This test also differentiates southern Spanish and South African colonies, which can be of great interest for the Africanized bee problem.
650	1	4	_aAPIS MELLIFERA
650	1	4	_aHONEYBEE
650	1	4	_aMITOCHONDRIAL DNA
650	1	4	_aRESTRICTION FRAGMENT LENGTH POLYMORPHISM
650	1	4	_aDRAI
650	1	4	_aPOLYMERASE CHAIN REACTION
650	1	4	_aCOI-COII INTERGENIC REGION
700	1	2	_aGarnery, L.
700	1	2	_aSolignac, M.
700	1	2	_aCelebrano, G.
700	1	2	_aCornuet, J.M.
856	4	0	_uhttps://drive.google.com/file/d/1VTafyzo6gOareoxaVENyvSNQSoRwFk5u/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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