000 01448nam a2200265Ia 4500
003 MX-MdCICY
005 20250625140650.0
040 _cCICY
090 _aB-11453
245 1 0 _adsRNA-induced gene silencing in Moniliophthora perniciosa, the causal agent of witches' broom disease of cacao
490 0 _vFungal genetics and Biology, 46(11), p.825-836, 2009
520 3 _aThe genome sequence of the hemibiotrophic fungus Moniliophthora perniciosa revealed genes possibly participating in the RNAi machinery. Therefore, studies were performed in order to investigate the efficiency of gene silencing by dsRNA. We showed that the reporter gfp gene stably introduced into the fungus genome can be silenced by transfection of in vitro synthesized gfpdsRNA. In addition, successful dsRNA-induced silencing of endogenous genes coding for hydrophobins and a peroxiredoxin were also achieved. All genes showed a silencing efficiency ranging from 18
700 1 2 _aCaribé Dos Santos A.C.
700 1 2 _aSena, J.A.
700 1 2 _aSantos, S.C,
700 1 2 _aDias, C.V.
700 1 2 _aPirovani, C.P.
700 1 2 _aPungartnik, C.
700 1 2 _aValle, R.R.
700 1 2 _aValle, R.R.
700 1 2 _aVincent, M.
856 4 0 _uhttps://drive.google.com/file/d/1n1chuY3M6_Gd8ZJ5MPntiCHSqjXMvaAU/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 _2Loc
_cREF1
008 250602s9999 xx |||||s2 |||| ||und|d
999 _c45669
_d45669