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090			_aB-11469
245	1	0	_aAxenic culture of Lemna trisulca L.
490	0		_vAquatic Botany, 38(2-3), p.295-301, 1990
520	3		_aMethods for sterilizing, subculturing and achieving sustained growth of Lemna trisulca L. in the laboratory are described. Successful growth and a doubling time of 1.9-2.0 days occurred using inorganic carbon at pH 7.8 as the sole carbon source under 400 µmol m-2s-1 cool white fluorescent light at 28±2°C. Cultures were aerated at a rate of 125 ml min-1 with sterile, humidified, room air. The growth medium was unbuffered and replaced once daily at an exponential rate based on the growth of the plant culture.
700	1	2	_aHueberta, David B.
700	1	2	_aMcilraitha, A.L.
700	1	2	_aShaya, J.M.
700	1	2	_aRobinson, G.G.C.
856	4	0	_uhttps://drive.google.com/file/d/16serHM7g5QQu0wXcyfzV1mvKF6uh06sJ/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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