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245 1 0 _aEffects of Substrate Diffusion and Anode Potential on Kinetic Parameters for Anode-Respiring Bacteria
490 0 _vEnvironmental Science & Technology, 43(19), p.7571-7577, 2009
520 3 _aThe substrate-utilization rate of anode-respiring bacteria (ARB)directly correlates to the current density, one of the main factors in a microbial electrolysis/fuel cell. This study first evaluates the effects of donor-substrate diffusion and anode potential on the estimation of the half-maximum-rate concentration (Ks)and the maximum specific substrate-utilization rate (qmax)of a mixed culture biofilm in a microbial electrolysis cell oxidizing acetate. The intrinsic Ks value is 119 g COD/m3, substrate diffusion has a significant impact on Ks estimation, and the effect of the anode potential on Ks is small. The intrinsic qmax value is 22.3 g COD/g VS-d for an assumed biomass density of 50,000 g VS/m3 (qmaxXf = 1120 kg COD/m3-d). The maximum specific growth rate (µmax)is 3.2/d which is substantially faster than for acetate-utilizing methanogens and homoacetogens. Although the anode potential affects qmax, substrate diffusion has a negligible effect. The measured half-saturation anode potential (EKA)is very negative, -0.448 V (vs Ag/AgCl), and this low value minimizes anode-potential limitation on the current density and the substrate-utilization rate. Thus, the ARB selected in our biofilm anode were relatively fast growers able to take advantage of their low EKA value (-0.448 V).
700 1 2 _aLee, Hyung-Sool.
700 1 2 _aTorres, Csar I.
700 1 2 _aRittmann, Bruce E.
856 4 0 _uhttps://drive.google.com/file/d/1olceMDJzlW2W8QRAQsLEB77obkzZqVWq/view?usp=drivesdk
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