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245	1	0	_aExtracellular proteomes of Arabidopsis thaliana and Brassica napus roots: analysis and comparison by MudPIT and LC-MS/MS
490	0		_vPlant Soil, 286, p.357-376, 2006
520	3		_aAn important principle of the functional organization of plant cells is the targeting of proteins to specific subcellular locations. The physical location of proteins within the apoplasm/ rhizosphere at the root-soil interface positions them to play a strategic role in plant response to biotic and abiotic stress. We previously demonstrated that roots of Triticum aestivum and Brassica napus exude a large suite of proteins to the apoplasm/rhizosphere [Basu et al. (1994)Plant Physiol 106:151-158; Basu et al. (1999)Physiol Plant 106:53-61]. This study is a first step to identify low abundance extracytosolic proteins from Arabidopsis thaliana and Brassica napus roots using recent advances in the field of proteomics. A total of 16 extracytosolic proteins were identified from B. napus using two-dimensional gel electrophoresis, tandem mass spectrometry (LC-MS/MS)and de novo sequencing. Another high-throughput proteomics approach, Multidimensional Protein Identification Technology (Mud PIT)was used to identify 52 extracytosolic proteins from A. thaliana. Signal peptide cleavage sites, the presence/absence of transmembrane domains and GPI modification were determined for these proteins. Functional classification grouped the extracellular proteins into different families including glycoside hydrolases, trypsin/ protease inhibitors, plastocyanin-like domains, copper-zinc superoxide dismutases, gamma-thioinins, thaumatins, ubiquitins, protease inhibitor/ seed storage/lipid transfer proteins, transcription factors, class III peroxidase, and plant basic secretory proteins (BSP). We have also developed an on-line, Extracytosolic Plant Proteins Database (EPPdb, http://eppdb.biology.ualberta. ca)to provide information about these extracytosolic proteins.
700	1	2	_aBasu, U.
700	1	2	_aFrancis, J.L.
700	1	2	_aWhittal, R.M.
700	1	2	_aStephens, J.L.
700	1	2	_aWang, Y.
700	1	2	_aZaiane, O.R.
700	1	2	_aGoebel, R.
700	1	2	_aGoebel, R.
700	1	2	_aTaylor, G.J.
856	4	0	_uhttps://drive.google.com/file/d/1wSwsfGRhvVvyzA9Z7EDzZ08QdAf1JW9u/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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