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090 _aB-11542
245 1 0 _aCharacterization of Fe-hydrogenase genes diversity and hydrogen-producing population in an acidophilic sludge
490 0 _vJournal of Biotechnology, 126(3), p.357-364, 2006
520 3 _aAccording to the DNA sequences of six Fe-hydrogenase genes (FHG)of Clostridium species retrieved from the GenBank, a set of primers specific for Fe-hydrogenase genes were identified from their common conserved regions. The length of DNA fragments amplified using these two primers averaged 313 bps. This primer set was then used to investigate the FHG diversity in an acidophilic rice-degrading sludge by methods based on polymerase chain reaction (PCR). Eight new Fe-hydrogenase gene fragments were identified from the sludge, as a result. Similarity based on amino acids among the 14 hydrogenase genes (8 newly found plus 6 known ones)was 39-97
650 1 4 _aACIDOPHILIC
650 1 4 _aFE-HYDROGENASE GENE
650 1 4 _aHYDROGEN PRODUCTION
650 1 4 _aPRIMER
650 1 4 _aQUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION
700 1 2 _aFang, H.H.P.
700 1 2 _aZhang, T.
700 1 2 _aLi, C.
856 4 0 _uhttps://drive.google.com/file/d/1PwjdVjfUMIZ5yv3rEl833-qNtxFTCVXJ/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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