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245 1 0 _aSynthesis of a Molecularly Imprinted Polymer for the Solid-phase Extraction of Betulin and Betulinic Acid from Plane Bark
490 0 _vPhytoChemical Analysis, 21(2), p.180-185, 2010
520 3 _aPlant extracts are usually complex mixtures of various polarity compounds and their study often includes a purifi cation step, such as solid-phase extraction (SPE), to isolate interest compounds prior analytical investigations. Molecularly imprinted polymers (MIPs)are a new promising type of SPE material which off er tailor-made selectivity for the extraction of trace active components in complex matrices. Numerous specifi c cavities that are sterically and chemically complementary of the target molecules, are formed in imprinted polymers. A molecularly imprinted polymer (MIP)was synthesised in order to trap a specifi c class of triterpene, including betulin and betulinic acid from a methanolic extract of plane bark. Methodology - Imprinted polymers were synthesised by thermal polymerisation of betulin as template, methacrylic acid (MAA)or acrylamide (AA)as functional monomer, ethylene glycol dimethacrylate as crosslinking agent and chloroform as porogen. Afterwards, MAA- and AA-MIPs were compared with their non-imprinted polymers (NIPs)in order to assess the selectivity vs betulin and its derivatives. Recovered triterpenes were analysed by HPLC during MIP-SPE protocol. Results - After SPE optimisation, the MAA-imprinted polymer exhibited highest selectivity and recovery (better than 70
650 1 4 _aMOLECULARLY IMPRINTED POLYMER
650 1 4 _aMIP
650 1 4 _aSOLID-PHASE EXTRACTION
650 1 4 _aSPE
650 1 4 _aBETULIN
650 1 4 _aBETULINIC ACID
650 1 4 _aTRITERPENE
650 1 4 _aPLANE BARK EXTRACT
700 1 2 _aClaude, B.
700 1 2 _aViron-Lamy, C.
700 1 2 _aHaupt, K.
700 1 2 _aMorin, P.
856 4 0 _uhttps://drive.google.com/file/d/1KY-I4CuFUF3r1iRzwDr1k9SIj7GbqTlB/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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