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090			_aB-11686
245	1	0	_aA Total Extract Dot Blot Hybridization Procedure for mRNA Quantitation in Small Samples of Tissues or Cultured Cells
490	0		_vAnalytical BioChemistry, 172(2), p.436-443, 1988
520	3		_aA simple method for the estimation of specific mRNA concentrations in small tissue samples (as little as 1 mg)or cultured cells (lower limit IO5 cells)is described. Guanidine hydrochloride extracts of whole cells or tissues are applied directly onto nitrocellulose and hybridized with the appropriate nick-translated probe. Loading according to DNA content allows expression ofthe result as concentration per cell. Hybridizing with a ribosomal RNA probe allows expression of results relative to rRNA and estimation of the RNA/DNA ratio in the sample. We describe the application of this procedure to the measurement of ceruloplasmin mRNA in tissues and cultured hepatocytes.
650	1	4	_aRECOMBINANT DNA TECHNOLOGY
650	1	4	_aMRNA
650	1	4	_aRNA: DNA: GENE EXPRESSION
700	1	2	_aGrimes, A.
700	1	2	_aMcardle, H.J.
700	1	2	_aMercer, J.F.B.
856	4	0	_uhttps://drive.google.com/file/d/1gwe3XIwUi-aNhBUWf2KiNkhVlGc3pnG_/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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