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090			_aB-11820
245	1	0	_aControl of microbial activity by flow injection analysis during high cell density cultivation of Escherichia coli
490	0		_vJournal of Biotechnology, 27(2), p.143-147, 1993
520	3		_aThe application of an automated flow injection analysis (FIA)system for on-line determination of microbial activity, during high cell density cultivations of Escherichia coli is reported. Based on a bioelectrochcmical principle, the FIA method used a redox mediator (potassium hexacyanoferrate(III)) to facilitate electron transfer from the microorganisms to an electrochemical detector. Assays were carried out using a new sampling device which provided aseptic operation by use of a valve and chemical sterilisation. No sample dilution or pretreatment was necessary for biomass concentrations up to approx. 40 g 1-1. The sample volume was 0.5 ml and the overall analysis time was 5 min. FIA signals were found to correlate well with the oxygen uptake rate (OUR). Changes in metabolic activity due to low substrate levels or high inhibitor concentrations in the cultivation medium became obvious from the FIA signals.
700	1	2	_aDing, T.
700	1	2	_aBilitewski, U.
700	1	2	_aSchmid, R.D.
700	1	2	_aKorz, D.J.
700	1	2	_aSanders, E.A.
856	4	0	_uhttps://drive.google.com/file/d/1t2jesEPOOYHaKjtVjhUS00OhG4kmL-xe/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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