000			02142nam a2200277Ia 4500
003			MX-MdCICY
005			20250625140701.0
040			_cCICY
090			_aB-12004
245	1	0	_aDetermination of the fraction of S-phase cells in root meristems using bromodeoxyuridine labeling
490	0		_vBiotechnic & HistoChemistry, 77(3)P.145-152, 2002
520	3		_aThe use of bromodeoxyuridine (BrdU)and subsequent immunocytochemical visualization for studying cell proliferation in plant meristems was investigated in Allium cepa L. root-tips. We describe the optimization of an indirect immunoperoxidase method for detecting incorporation of this DNA precursor in pulse-labeled cells. The basic object of this study is to quantify the extent to which the fraction of S-phase cells can reliably be estimated in asynchronous populations. A matrix of parallel labeling schedules with tritiated-thymidine or BrdU was developed, and the labeling indices provided by autoradiography or immunocytochemistry were compared. Thus, 0.5 mM BrdU assured saturation S-phase labeling after an exposure time of 30 min, and the mean length of the S-phase determined under such conditions was similar to that previously reported for this plant system. Interestingly, Feulgen staining did not interfere with subsequent detection of the BrdU probe. This allowed comparative evaluations of the nuclear DNA content by Feulgenmicrodensitometry and the position of a given cell in G1, S or G2 compartments. We also explored the possibility of quantifying BrdU-incorporation in single nuclei by densitometry measurement of the peroxidase label.
650	1	4	_aALLIUM CEPA
650	1	4	_aBROMODEOXYURIDINE
650	1	4	_aCELL CYCLE
650	1	4	_aIMMUNOPEROXIDASE
650	1	4	_aMERISTEMS
650	1	4	_aMICRODENSITOMETRY
650	1	4	_aS-PHASE
700	1	2	_aHervas, Jp
700	1	2	_aFlor, J.
700	1	2	_aSanta-Cruz, M.C.
856	4	0	_uhttps://drive.google.com/file/d/1oYruqEFDqO9NCzyW3ujGUhp__dHsIx0u/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c46217 _d46217