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245	1	0	_aRapid cDNA synthesis and sequencing techniques for the genetic study of bluetongue and other dsRNA viruses
490	0		_vJournal of Virological Methods, 143(2), p.132-139, 2007
520	3		_aThe genetic study of double-stranded (ds)RNA viruses by sequence analyses of full-length genome segments, or entire viral genomes, has been restricted by the technical difficulties involved in analyses of dsRNA templates. This paper describes improved methods for sequence-independent synthesis of full-length cDNA copies of dsRNA genes and associated sequencing strategies. These methods include an improved version of the 'Single Primer Amplification Technique' (SPAT - [Attoui, H., Billoir, F., Cantaloube, J.F., Biagini, P., de Micco, P. and de Lamballerie, X., 2000. Strategies for the sequence determination of viral dsRNA genomes. J. Virol. Methods 89, 147-158]), which is described here as 'Full-Length Amplification of cDNAs' (FLAC). They also include the development of direct sequencing methods (without cloning)for the resulting full-length cDNAs. These techniques, which are applicable to any viruses with segmented dsRNA genomes and conserved RNA termini, make it possible to generate sequence data rapidly from multiple isolates for molecular epidemiology studies.
650	1	4	_aBLUETONGUE VIRUS
650	1	4	_aREOVIRUS
650	1	4	_aDSRNA VIRUS
650	1	4	_aCDNA
650	1	4	_aRAPID SEQUENCING
650	1	4	_aFLAC
700	1	2	_aMaan, S.
700	1	2	_aRao, S.
700	1	2	_aSingh Maan, N.
700	1	2	_aAnthony, S.J.
700	1	2	_aAttoui, H.
700	1	2	_aRichard Samuel, A.
700	1	2	_aClement Mertens, P.P.
856	4	0	_uhttps://drive.google.com/file/d/1A8Afrihvr6WCUtYc-IUHoHr-fbl1QDay/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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