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090			_aB-12260
245	1	0	_aConstruction of two Gateway vectors for gene expression in fungi
490	0		_vPlasmid, 62(2), p.128-133, 2009
520	3		_aWe report the construction of two Gateway fungal expression vectors pCBGW and pGWBF. The pCBGW was generated by introducing an expression cassette, which consists of a Gateway recombinant cassette (attR1-Cmr-ccdB-attR2)under the control of fungal promoter PgpdA and a terminator TtrpC, into the multiple cloning site of fungal vector pCB1004. The pGWBF is a binary vector, which was generated from the plant expression vector pGWB2 by replacing the CaMV35S promoter with PgpdA. The pGWBF can be transformed into fungi efficiently with Agrobacterium-mediated transformation. The applicability of two newly constructed vectors was tested by generating the destination vectors pGWBF-GFP and pCBGW-GFP and examining the expression of GFP gene in Trichoderma viride and Gibberella fujikuroi, respectively. Combining with the advantage of Gateway cloning technology, pCBGW and pGWBF will be useful in fungi for large-scale investigation of gene functions by constructing the interested gene destination/expression vectors in a highthroughput way
650	1	4	_aFUNGAL EXPRESSION VECTOR
650	1	4	_aGATEWAY CLONING TECHNOLOGY
650	1	4	_aAGROBACTERIUM-MEDIATED TRANSFORMATION
700	1	2	_aZhu, T.
700	1	2	_aWang, W.
700	1	2	_aYang, X.
700	1	2	_aWang, K.
700	1	2	_aCui, Z.
856	4	0	_uhttps://drive.google.com/file/d/1hKtyL-T2fdN5WJbWYfb1OnJCtvmg1PuS/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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