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090			_aB-12284
245	1	0	_aBacterial diversity of soils assessed by DGGE, T-RFLP and SSCP fingerprints of PCR-amplified 16S rRNA gene fragments: Do the different methods provide similar results?
490	0		_vJournal of MicroBiological Methods, 69(3), p.470-479, 2007
520	3		_aBacterial communities of four arable soils - pelosol, gley, para brown soil, and podsol brown soil - were analysed by fingerprinting of 16S rRNA gene fragments amplified from total DNA of four replicate samples for each soil type. Fingerprints were generated in parallel by denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP), and single strand conformation polymorphism (SSCP)to test whether these commonly applied techniques are interchangeable. PCR amplicons could be separated with all three methods resulting in complex ribotype patterns. Although the fragments amplified comprised different variable regions and lengths, DGGE, T-RFLP and SSCP analyses led to similar findings: (a)a clustering of fingerprints which correlated with soil physico-chemical properties, (b)little variability between the four replicates of the same soil, (c)the patterns of the two brown soils were more similar to each other than to those of the other two soils, and (d)the fingerprints of the different soil types revealed significant differences in a permutation test, which was recently developed for this purpose.
650	1	4	_a16S RRNA GENE
650	1	4	_aDGGE
650	1	4	_aSOIL BACTERIAL DIVERSITY
650	1	4	_aSSCP
650	1	4	_aTOTAL COMMUNITY DNA
650	1	4	_aT-RFLP
700	1	2	_aSmalla, K.
700	1	2	_aOros-Sichler, M.
700	1	2	_aMilling, A.
700	1	2	_aHeuer, H.
700	1	2	_aBaumgarte, S.
700	1	2	_aBecker, R.
700	1	2	_aNeuber, G.
700	1	2	_aNeuber, G.
700	1	2	_aUlrich, A.
700	1	2	_aTebbe, C.C.
856	4	0	_uhttps://drive.google.com/file/d/1nxTZaDuY5hvuOjFQLpBlHeFaif3XrsPQ/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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