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003			MX-MdCICY
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090			_aB-12292
245	1	0	_aA strategy for rapid cDNA cloning from double-stranded RNA templates isolated from plants infected with RNA viruses by using Taq DNA polymerase
490	0		_vJournal of Virological Methods, 84(1), p.59-63, 2000
520	3		_aA fast and efficient cDNA cloning procedure for plant RNA viruses was developed. In this procedure, doublestranded RNA (dsRNA)was used as a template source. Standard cDNA synthesis reagents and random hexamers were then used for making cDNAs. Taq DNA polymerase was used to add additional (A)at the ends of cDNAs, a TA cloning kit to ligate the cDNAs to vectors, and an electroporator to transform the DNAs to E. coli cells. dsRNAs were extracted from grapevine tissues infected with four different viruses and used for cloning. These viruses included grapevine rupestris stem pitting associated virus, grapevine leafroll associated virus 5, and two uncharacterized grapevine viruses, one each closely related to marafivirus and vitivirus groups. Selected cDNA clones were sequenced and PCR primers were developed for RT-PCR detection of these viruses in host plants.
650	1	4	_aGRAPEVINE VIRUS
650	1	4	_aVIRUS DETECTION
650	1	4	_aPCR
650	1	4	_aCDNA CLONING
650	1	4	_aDSRNA
700	1	2	_aZhang, Y.
700	1	2	_aRowhani, A.
856	4	0	_uhttps://drive.google.com/file/d/1m_V9d5h0J4F9PJYlXf_5Pz_ANaejP7cs/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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