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090 _aB-12364
245 1 0 _aIncreased glutamine synthetase by overexpression of TaGS1 improves grain yield and nitrogen use efficiency in rice
490 0 _vPlant Physiology and BioChemistry, 169, p.259-268, 2021
520 3 _aImproving nitrogen use efficiency (NUE)has been a focal point for crop growth and yield throughout the world. Glutamine synthetase (GS), which plays a fundamental role in N metabolism, has been exploited to improve crop NUE. However, increased GS activity in rice by overexpressing its own GS genes hasn't shown superior plant productivity. Here, transgenic rice plants with increased GS activity by overexpressing TaGS1 were analyzed under field and culture conditions at two N rates. Transgenic expression of TaGS1 significantly increases GS activity in leaves, junctions and roots of rice plants relative to wide-type plants. When rice plants grown under consecutive field trials with N rates of 60 and 240 kg/ha, three transgenic lines have higher grain yield than wild-type plants, with increment of 15 percent-22 percent in T2 generation and with that of 28 percent-36 percent in T3 generation, respectively. And increased panicle numbers (effective tiller numbers)mainly contribute to the advantage of grain yield in transgenic plants. Analysis of N use-related traits shows that transgenic plants with enhanced GS activity promote root capacity to obtain N, N accumulation during growth stages and N remobilization to grains, ultimately conferring 31 percent-40 percent improvement of NUE relative to wild-type rice plants.
650 1 4 _aRICE
650 1 4 _aGLUTAMINE SYNTHETASE
650 1 4 _aNITROGEN USE EFFICIENCY
700 1 2 _aWu, D.
700 1 2 _aLi, Y.
700 1 2 _aCao, Y.
700 1 2 _aHu, R.
700 1 2 _aWu, X.
700 1 2 _aZhang, W.
700 1 2 _aZhang, Y.
856 4 0 _uhttps://drive.google.com/file/d/1UpKG0m82-df_4rMZ4KNzKYRBfix-yI8Y/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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