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245 1 0 _aCharacterization and partial purification of acyl-CoA:glycerol 3-phosphate acyltransferase from sunflower (Helianthus annuus L.)developing seeds
490 0 _vPlant Physiology and BioChemistry, 48(2-3), p.73-80, 2010
520 3 _aThe glycerol 3-phosphate acyltransferase (GPAT, EC 2.3.1.15)from sunflower (Helianthus annuus L.)microsomes has been characterised and partially purified. The in vitro determination of activity was optimized, and the maximum value for GPAT activity identified between 15 and 20 days after flowering. The apparent MichaeliseMenten Km for the glycerol 3-phosphate was 354 mM. The preferred substrates were palmitoyl-CoA ¼ linoleoyl-CoA > oleoyl-CoA with the lowest activity using stearoyl-CoA. High solubilisation was achieved using 0.75
650 1 4 _aACYLTRANSFERASE
650 1 4 _aGLYCEROL 3-PHOSPHATE
650 1 4 _aHELIANTHUS ANNUUS
650 1 4 _aMICROSOMAL MEMBRANE
650 1 4 _aSUNFLOWER
650 1 4 _aSEEDS
700 1 2 _aRuiz-López, N.
700 1 2 _aGarcés, R.
700 1 2 _aHarwood, J.L.
700 1 2 _aMartínez-Force, E.
856 4 0 _uhttps://drive.google.com/file/d/19Yjctt8_Fgxy4-dPwM-cxQx2AG8xASuw/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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