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| 005 | 20250625153854.0 | ||
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| 090 | _aB-12476 | ||
| 245 | 1 | 0 | _aSynthesis activity-based zymography for detection of lipases and esterases |
| 490 | 0 | _vBiotechnol Lett, DOI 10.1007/s10529-010-0485-4, 2010 | |
| 520 | 3 | _aA new zymography method for lipases and esterases was developed on the basis of the esterification reaction between fatty acids and alcohols. The enzymes were separated by SDS-PAGE and native PAGE. The gel was washed and then incubated in an aqueous solution containing fatty acids (oleic acid 18:1 or caprylic acid 8:0)and dodecanol. Synthesis was visualized by in situ precipitation of water-insoluble and non-diffusible fatty acid esters, such as dodecyl oleate and dodecyl octanoate. The synthesis activity-based zymography was confirmed with different enzyme samples, including commercial lipase preparations, purified recombinant lipase and cutinase, and crude culture supernatants of lipolytic enzyme-producing soil bacteria. | |
| 650 | 1 | 4 | _aESTERIFICATION |
| 650 | 1 | 4 | _aFATTY ACID ESTER |
| 650 | 1 | 4 | _aLIPASE |
| 650 | 1 | 4 | _aSYNTHESIS ACTIVITY |
| 650 | 1 | 4 | _aZYMOGRAPHY |
| 700 | 1 | 2 | _aKwon, M. |
| 700 | 1 | 2 | _aKim, H.S. |
| 700 | 1 | 2 | _aHahm, D. |
| 700 | 1 | 2 | _aSong, J.K. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1PG0KppIMp3JQ-iaBIFhAK_OWvtb-j_se/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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