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090			_aB-12481
245	1	0	_aArtificial inoculation on plants and banana leaf pieces with Mycosphaerella spp., responsible for Sigatoka leaf spot diseases
490	0		_vFruits, 63(5), p.319-323, 2008
520	3		_aThis protocol aims at (i)analysing the nature of host-parasite interactions (resistance or susceptibility), and (ii)studying the variability of the pathogenicity of different pathogen populations (intra/inter-specific)with regard to both virulence and aggressiveness using artificial inoculation with Mycosphaerella fijiensis or Mycosphaerella musicola on plants and banana leaf pieces. The principle, key advantages, starting plant material and time required for the method are presented. Materials and methods. Laboratory materials required and details of the sixteen steps of the protocol (conidial inoculum preparation, artificial inoculation on banana plants and artificial inoculation on banana leaf pieces)are described. Possible troubleshooting is discussed. Results. Typical symptoms of the different diseases are obtained after artificial inoculation.
650	1	4	_aMUSA SP.
650	1	4	_aMYCOSPHAERELLA FIJIENSIS
650	1	4	_aMYCOSPHAERELLA MUSICOLA
650	1	4	_aDISEASE RESISTANCE
650	1	4	_aMETHODS
700	1	2	_aAbadie, C.
700	1	2	_aZapater, M.F.
700	1	2	_aPignolet, L.
700	1	2	_aCarlier, J.
700	1	2	_aMourichon, X.
856	4	0	_uhttps://drive.google.com/file/d/1XFooEgillg1sl3Y10JKfs43U-rkjekne/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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