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090			_aB-12499
245	1	0	_aGenetically pyramiding protease-inhibitor genes for dual road-spectrum resistance against insect and phytopathogens in transgenic tobacco
490	0		_vPlant Biotechnology Journal, 8(1), p.65-75, 2010
520	3		_aProtease inhibitors provide a promising means of engineering plant resistance against attack by insects and pathogens. Sporamin (trypsin inhibitor)from sweet potato and CeCPI (phytocystatin)from taro were stacked in a binary vector, using pMSPOA (a modified sporamin promoter)to drive both genes. Transgenic tobacco lines of T0 and T1 generation with varied inhibitory activity against trypsin and papain showed resistance to both insects and phytopathogens. Larvae of Helicoverpa armigera that ingested tobacco leaves either died or showed delayed growth and development relative to control larvae. Transgenic tobacco-overexpressing the stacked genes also exhibited strong resistance against bacterial soft rot disease caused by Erwinia carotovora and damping-off disease caused by Pythium aphanidermatum. Thus, stacking protease-inhibitor genes, driven by the wound and pathogen responsive pMSPOA promoter, is an effective strategy for engineering crops to resistance against insects and phytopathogens.
650	1	4	_aSPORAMIN
650	1	4	_aCECPI (TARO CYSTATIN)
650	1	4	_aSYNTHETIC WOUND-INDUCIBLE&PROMOTER (PMSPOA)
650	1	4	_aTRANSGENIC TOBACCO
650	1	4	_aINSECT RESISTANCE
650	1	4	_aANTIBACTERIAL ACTIVITY
650	1	4	_aANTIFUNGAL ACTIVITY
700	1	2	_aSenthilkumar, R.
700	1	2	_aCheng, C.
700	1	2	_aYeh, K.
856	4	0	_uhttps://drive.google.com/file/d/1Ela5eGXdmJXX1d2Y7CVTl2K2Nvu6oXT6/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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