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245	1	0	_aGenome-wide mapping of Arabidopsis thaliana origins of DNA replication and their associated epigenetic marks
490	0		_vNature Structural & Molecular Biology, 18, p.395-400, 2011
520	3		_aGenome integrity requires faithful chromosome duplication. Origins of replication, the genomic sites at which DNA replication initiates, are scattered throughout the genome. Their mapping at a genomic scale in multicellular organisms has been challenging. In this study we profiled origins in Arabidopsis thaliana by high-throughput sequencing of newly synthesized DNA and identified ~1,500 putative origins genome-wide. This was supported by chromatin immunoprecipitation and microarray (ChIP-chip)experiments to identify ORC1- and CDC6-binding sites. We validated origin activity independently by measuring the abundance of nascent DNA strands. The midpoints of most A. thaliana origin regions are preferentially located within the 5_O half of genes, enriched in G+C, histone H2A.Z, H3K4me2, H3K4me3 and H4K5ac, and depleted in H3K4me1 1 and H3K9me2. Our data help clarify the epigenetic specification of DNA replication origins in A. thaliana and have implications for other eukaryotes.
700	1	2	_aCostas, C.
700	1	2	_aSanchez, M.
700	1	2	_aStroud, H.
700	1	2	_aYu, Y.
700	1	2	_aOliveros, J.C.
700	1	2	_aFeng, S.
700	1	2	_aBenguria, A.
700	1	2	_aBenguria, A.
700	1	2	_aZhang, X.
700	1	2	_aSolano, R.
700	1	2	_aJacobsen, S.E.
700	1	2	_aGutierrez, C.
856	4	0	_uhttps://drive.google.com/file/d/1J7YDYNbMLA6RRqUOEeyZkSlkpqYQXSAU/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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