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245	1	0	_aFormation of embryogenic cell clumps from carrot epidermal cells is suppressed by 5-azacytidine, a DNA methylation inhibitor
490	0		_vJournal of Plant Physiology, 162(1), p.47-54, 2005
520	3		_aUsing a direct somatic embryogenesis system in carrot, we examined the role of DNA methylation in the change of cellular differentiation state, from somatic to embryogenic. 5-Azacytidine (aza-C), an inhibitor of DNA methylation suppressed the formation of embryogenic cell clumps from epidermal carrot cells. Aza-C also downregulated the expression of DcLEC1c, a LEC1-like embryonic gene in carrot, during morphogenesis of embryos. A carrot DNA methyltransferase gene, Met1-5 was expressed transiently after the induction of somatic embryogenesis by 2,4- dichlorophenoxyacetic acid (2,4-D), before the formation of embryogenic cell clumps. These findings suggested the significance of DNA methylation in acquiring the embryogenic competence in somatic cells in carrot.
650	1	4	_a5-AZACYTIDINE
650	1	4	_aCARROT
650	1	4	_aDNA METHYLATION
650	1	4	_aSOMATIC EMBRYOGENESIS
700	1	2	_aYamamoto, N.
700	1	2	_aKobayashi, H.
700	1	2	_aTogashi, T.
700	1	2	_aMori, Y.
700	1	2	_aKikuchi, K.
700	1	2	_aKuriyama, K.
700	1	2	_aTokuji, Y.
856	4	0	_uhttps://drive.google.com/file/d/1D3mVPafMd6zEKOw2YTuV1-qcZLPGQO9s/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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