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090			_aB-12759
245	1	0	_aOn the art of identifying effective and specific siRNAs
490	0		_vNature Methods, 3, p.670-676, 2006
520	3		_aSmall interfering RNAs (siRNAs)have been widely exploited for sequence-specific gene knockdown, predominantly to investigate gene function in cultured vertebrate cells, and also hold promise as therapeutic agents. Because not all siRNAs that are cognate to a given target mRNA are equally effective, computational tools have been developed based on experimental data to increase the likelihood of selecting effective siRNAs. Furthermore, because target-complementary siRNAs can also target other mRNAs containing sequence segments that are partially complementary to the siRNA, most computational tools include ways to reduce potential off-target effects in the siRNA selection process. Though these methods facilitate selection of functional siRNAs, they do not yet alleviate the need for experimental validation. This perspective provides a practical guide based on current wisdom for selecting siRNAs.
700	1	2	_aPei, Y.
700	1	2	_aTuschl, T.
856	4	0	_uhttps://drive.google.com/file/d/1TbyPMAJZKd9oK6pXKvMFIrg_smqjaotY/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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