| 000 | 01915nam a2200313Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20250625153908.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-12762 | ||
| 245 | 1 | 0 | _aAn Improved Protocol for Coupling Synthetic Peptides to Carrier Proteins for Antibody Production Using DMF to Solubilize Peptides |
| 490 | 0 | _vJournal of BioMolecular Techniques, 18(3), p.173-176, 2007 | |
| 520 | 3 | _aWe present an improved protocol for coupling synthetic peptides to carrier proteins. In this protocol, dimethylformamide is used as the solvent to solubilize peptides instead of phosphate-buffered saline (PBS)or 6 M guanidine-HC l/0.01 M phosphate buffer (pH 7). Additionally, the last desalting or dialyzing step to remove uncoupled peptides as in the traditional method is eliminated. Finally, 3 mL of 0.1 M ammonium bicarbonate is added to the carrier protein conjugated peptide solution to help the lyophilization process. Coupling of Cys-containing synthetic peptides to keyhole limpet hemocyanin or bovine serum albumin using mmaleimidobenzoyl- N-hydroxysuccinimide ester are used as the test cases. This method produces high-quality antipeptide antibodies. Also, compared to the traditional method, this procedure is simpler and useful for peptides with solubility problems in PBS or 6 M guanidine-HC l. | |
| 650 | 1 | 4 | _aPEPTIDE |
| 650 | 1 | 4 | _aANTIBODY |
| 650 | 1 | 4 | _aKLH |
| 650 | 1 | 4 | _aBSA |
| 650 | 1 | 4 | _aMBS |
| 650 | 1 | 4 | _aDMF |
| 650 | 1 | 4 | _aMALDI- TOF MS |
| 700 | 1 | 2 | _aSyed Salman Lateef |
| 700 | 1 | 2 | _aShalini Gupta |
| 700 | 1 | 2 | _aLasanthi P. Jayathilaka |
| 700 | 1 | 2 | _aSangeeth Krishnanchettiar |
| 700 | 1 | 2 | _aJin-Sheng Huang |
| 700 | 1 | 2 | _aBao-Shiang Lee |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1qQBDR1MayE9oarTAoX-ADGzzSYtE4b2l/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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