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245 1 0 _aInfluence of NaCl on the Growth and Metabolism of Halomonas salina
490 0 _vJ. Microbiol. Biotechnol., 15(1), p.118-124, 2005
520 3 _aIn this research, we examined the effect of NaCl on the growth, energy metabolism, and proton motive force of Halomonas salina, and the effect of compatible solutes on the bacterium growing in the high salinity environment. H. salina was isolated from seawater and identified by 16srDNA sequencing. The growth of H. salina was not nhanced by the addition of external compatible solutes (choline and betaine)in the high salinity environment. The resting cells of H. salina absorbed more glucose in the presence of 2.0 M NaCl than in its absence. H. salina did not grow in the medium with either KCl, RbCl, CsCl, Na2SO4, or NaNO3, in place of NaCl. The optimal concentration of NaCl for the growth of H. salina ranged from 1.4 M to 2.5 M, and the growth yield was decreased in the presence of NaCl below 1.4M and above 2.5M. The activity of isocitrate dehydrogenase, pyruvate dehydrogenase, and malate dehydrogenase of H. salina was not inhibited by NaCl in in vitro test. The proton translocation of H. salina was detected in the presence of NaCl only. These results indicate that NaCl is absolutely required for the normal growth and energy metabolism of H. salina, but the bacterial growth is not enhanced by the compatible solutes added to the growth medium.
650 1 4 _aHALOMONAS SALINA
650 1 4 _aCOMPATIBLE SOLUTE
650 1 4 _aPROTON TRANSLOCATION
650 1 4 _aSALINITY ENVIRONMENT
650 1 4 _aCYCLIC VOLTAMMETRY
650 1 4 _aHALOPHILIC BACTERIUM
700 1 2 _aYun, Su Hee
700 1 2 _aSang Byung In
700 1 2 _aDoo Hyun Park
856 4 0 _uhttps://drive.google.com/file/d/1K7k23Z6lHC7GirewFfoO_aSlBtXtcMEW/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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