000			01448nam a2200193Ia 4500
003			MX-MdCICY
005			20250625153913.0
040			_cCICY
090			_aB-12986
245	1	0	_aMolecular Beacons: A Real-Time Polymerase Chain Reaction Assay for Detecting Salmonella
490	0		_vAnalytical BioChemistry, 280(1), p.166-172, 2000
520	3		_aMolecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a real-time PCR assay to detect the presence of Salmonella species using these fluorogenic reporter molecules. A 122-base-pair section of the himA was used as the amplification target. Molecular beacons were designed to recognize a 16-base-pair region on the amplicon. As few as 2 colony-forming unit (CFU)per PCR reaction could be detected. We also demonstrated the ability of the molecular beacons to discriminate between amplicons obtained from similar species such as Escherichia coli and Citrobacter freundii in realtime PCR assays. These assays could be carried out entirely in sealed PCR tubes, enabling fast and direct detection of Salmonella in a semiautomated format
700	1	2	_aChen, W.
700	1	2	_aMartinez, G.
700	1	2	_aMulchandani, A.
856	4	0	_uhttps://drive.google.com/file/d/1CBNx9B8h8veSAh2it0A0kCdPZqGxqLv1/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c47188 _d47188