| 000 | 01354nam a2200337Ia 4500 | ||
|---|---|---|---|
| 003 | MX-MdCICY | ||
| 005 | 20250625153913.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-12992 | ||
| 245 | 1 | 0 | _aProtoplast Transformation of Filamentous Fungi |
| 490 | 0 | _vMolecular and Cell Biology Methods for Fungi, Methods in Molecular Biology, 638, p.3-19, 2010 | |
| 520 | 3 | _aThe protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI)procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed. | |
| 650 | 1 | 4 | _aSELECTABLE MARKER |
| 650 | 1 | 4 | _aDRUG RESISTANCE |
| 650 | 1 | 4 | _aSPLIT-MARKER |
| 650 | 1 | 4 | _aGENE-DELETION |
| 650 | 1 | 4 | _aCOTRANSFORMATION |
| 650 | 1 | 4 | _aHYGROMYCIN B |
| 650 | 1 | 4 | _aGENETICIN |
| 650 | 1 | 4 | _aBIALAPHOS |
| 650 | 1 | 4 | _aPHOSPHOTHRICIN |
| 650 | 1 | 4 | _aREMI |
| 650 | 1 | 4 | _aINTEGRATION |
| 700 | 1 | 2 | _aTurgeon, B.G. |
| 700 | 1 | 2 | _aCondon, B. |
| 700 | 1 | 2 | _aLiu, J. |
| 700 | 1 | 2 | _aZhang, N. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/10XWa9Prehse4f3jSjl1EeaELw7Cr_uq0/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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