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245	1	0	_aDifferential screening reveals genes differentially expressed in low- and high-virulence near isogenic Phytophthora sojae lines
490	0		_vFungal Genetics and Biology, 43(12), p.826-839, 2006
520	3		_aTo explore the molecular mechanisms involved in virulence variations in Phytophthora sojae, the low-virulence isolate PS2 was inoculated successively on a resistant soybean (Glycine max)cultivar. After 14 successive inoculations, a high-virulence progeny, termed PS2- vir, was obtained and demonstrated to exhibit lower oospore production. DNA Wngerprinting revealed no large-scale DNA diVerences in PS2 and PS2-vir. A suppression subtractive hybridization (SSH)approach was developed to investigate diVerences in gene expression between PS2 and PS2-vir in the early stages of soybean infection. Of the 323 sequences chosen for examination, 74 utative unigenes were identiWed that exhibit high expression in PS2-vir. These sequences are predicted to encode proteins involved in energy production, protein biosynthesis, cell signaling, cell-wall biogenesis, and transcription regulation. Ten clones were selected for temporal expression analysis using RT-PCR based on the results of the dot-blot screens. The possible genetic mechanisms involved in these phenomena are discussed.
650	1	4	_aPHYTOPHTHORA SOJAE
650	1	4	_aTRANSCRIPTION REGULATION
650	1	4	_aVIRULENCE VARIATION
650	1	4	_aOOSPORE PRODUCTION
700	1	2	_aZiying, W.
700	1	2	_aYuanchao, W.
700	1	2	_aXiaoren, C.
700	1	2	_aGui, S.
700	1	2	_aZhengguang, Z.
700	1	2	_aXiaobo, Z.
856	4	0	_uhttps://drive.google.com/file/d/1F3wztM52KGN0YW67anGpMrwz-BVKv4gT/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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