000			01439nam a2200181Ia 4500
003			MX-MdCICY
005			20250625153915.0
040			_cCICY
090			_aB-13080
245	1	0	_aArabidopsis thaliana as a model system in somatic cell genetics II. Cell suspension culture
490	0		_vPlant Science Letters, 8, p.7-15, 1977
520	3		_aA cell culture system providing a suitable source of free cells for in vitro genetic manipulations is presented. The system is based on the correlation between dissociation rate and growth pattern in suspension cultures. This correlation is used to control the balance between cell division and cell dissociation. 2,4-Dichlorophenoxyacetic acid (2,4-D)at a concentration of 1 mg/1 is estimated to be the most efficient dissociating factor. The culture is permanently maintained in the late log phase by adding fresh medium to the biomass at a well-defined ratio; thus, cells are kept growing and cell dissociation is favoured. This makes it possible to use large cell aggregates s a continuous source of single cells and of small cell aggregates. A yield of 0.8 to 1.8.106 viable cells per ml is obtained.
700	1	2	_aNegrutiu, I.
700	1	2	_aJacobs, M.
856	4	0	_uhttps://drive.google.com/file/d/1EBQ6ldUeRMhOwInJ9iM8Rrq8VibxH5Kl/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c47282 _d47282