000			02274nam a2200385Ia 4500
003			MX-MdCICY
005			20250625153916.0
040			_cCICY
090			_aB-13122
245	1	0	_aHuman single-donor composite skin substitutes based on collagen and polycaprolactone copolymer
490	0		_vBioChemical and Biophysical Research Communications, 386(1), p.21-25, 2009
520	3		_aThe development and characterization of an enhanced composite skin substitute based on collagen and poly(e-caprolactone)are reported. Considering the features of excellent biocompatibility, easy-manipulated property and exempt from cross-linking related toxicity observed in the 1:20 biocomposites, skin substitutes were developed by seeding human single-donor keratinocytes and fibroblasts alone on both sides of the 1:20 biocomposite to allow for separation of two cell types and preserving cell signals transmission via micro-pores with a porosity of 28.8 ± 16.1 lm. The bi-layered skin substitute exhibited both differentiated epidermis and fibrous dermis in vitro. Less Keratinocyte Growth Factor production was measured in the co-cultured skin model compared to fibroblast alone condition indicating a favorable microenvironment for epidermal homeostasis. Moreover, fast wound closure, epidermal differentiation, and abundant dermal collagen deposition were observed in composite skin in vivo. In summary, the beneficial characteristics of the new skin substitutes exploited the potential for pharmaceutical screening and clinical application.
650	1	4	_aSKIN SUBSTITUTE
650	1	4	_aCOLLAGEN
650	1	4	_aPOLY(E-CAPROLACTONE)
650	1	4	_aBIOCOMPOSITE
650	1	4	_aKERATINOCYTE
650	1	4	_aFIBROBLAST
650	1	4	_aPOROSITY
700	1	2	_aDai, N.
700	1	2	_aYeh, M.
700	1	2	_aChiang, C.
700	1	2	_aChen, K.
700	1	2	_aLiu, T.
700	1	2	_aFeng, A.
700	1	2	_aChao, L.
700	1	2	_aChao, L.
700	1	2	_aSytwu, H.
700	1	2	_aChen, S.
700	1	2	_aChen, T.
700	1	2	_aAdams, E.F.
856	4	0	_uhttps://drive.google.com/file/d/1w3No6w8jfRCkbjHWlCN_G3GPcnXJM4Mf/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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