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090			_aB-13182
245	1	0	_aAssessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis
490	0		_vJournal of MicroBiological Methods, 75(2), p.302-307, 2008
520	3		_aXylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X. fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 106 to 104 bacterial cells, the IC-PCR sensitivity was up to 103 and the detection limit of I-PCR was up to 101 bacterial cells. These methods can use either plant sample extracts or cultivated media, and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X. fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC.
650	1	4	_aXYLELLA FASTIDIOSA
650	1	4	_aCITRUS VARIEGATED CHLOROSIS
650	1	4	_aIMMUNOCAPTURE-PCR
650	1	4	_aIMMUNO-PCR
650	1	4	_aELISA
650	1	4	_aPCR
700	1	2	_aPeroni, L.A.
700	1	2	_aRibeiro Dos Reis, J.R.
700	1	2	_aColetta-Filho, H.D.
700	1	2	_aAlves De Souza, A.
700	1	2	_aMachado, M.A.
700	1	2	_aStach-Machado, D.R.
856	4	0	_uhttps://drive.google.com/file/d/1tA63hQoQMhooVIB3mkxuJOGe8-irs8vz/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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