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090			_aB-13267
245	1	0	_aSpectrofluorimetric method for the estimation of total lipids in Eremothecium ashbyii fungal filaments using Nile blue and avoiding interference of autofluorescent riboflavin
490	0		_vJournal of MicroBiological Methods, 55(1), p.99-103, 2003
520	3		_aA rapid, simple and sensitive spectrofluorimetric technique was developed for monitoring total lipids in hyphae of the riboflavin-overproducing fungus Eremothecium ashbyii using the fluorescent probe Nile blue in an aqueous system, avoiding the interference due to autofluorescent riboflavin. The existing methodologies for lipid estimation are tedious, requiring large biomass, solvent extraction and gravimetry. E. ashbyii is a hemiascomycete fungus which accumulates lipids in its mycelia prior to flavinogenesis. This study defines the conditions (wavelength selection and sensitivity)for the spectrofluorimetric quantification of lipids in situ in the macerated mycelia of this fungus in the presence of intracellular autofluorescent riboflavin without the need to extract the lipids from the mycelia. The fluorescent intensity was linear with the lipid concentration of the mycelia (by gravimetry)under three different growth conditions using glucose, olive oil and sunflower oil as carbon sources. This spectrofluorimetic method of lipid estimation can be applied to other fungi and microorganisms.
650	1	4	_aNILE BLUE
650	1	4	_aPHENOXAZINE
650	1	4	_aIN SITU LIPID ESTIMATION
650	1	4	_aFLUORESCENT PROBE
650	1	4	_aSPECTROFLUORIMETRY
650	1	4	_aEREMOTHECIUM ASHBYII
650	1	4	_aMYCELIA
650	1	4	_aRIBOFLAVIN
650	1	4	_aGRAVIMETRY
700	1	2	_aVijayalakshmi, S.
700	1	2	_aKarthika, T.N.
700	1	2	_aMishra, A.K.
700	1	2	_aChandra, T.S.
856	4	0	_uhttps://drive.google.com/file/d/1DAK4yNJwdr4P-JzjuzKJNOjJP57YA4iO/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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