000			01945nam a2200265Ia 4500
003			MX-MdCICY
005			20250625153922.0
040			_cCICY
090			_aB-13407
245	1	0	_aA novel staining method for detecting phytase activity
490	0		_vJournal of MicroBiological Methods, 39(1), p.17-22, 1999
520	3		_aDifferential agar media for the detection of microbial phytase activity use the disappearance of precipitated calcium or sodium phytate as an indication of enzyme activity. When this technique was applied to the study of ruminal bacteria, it became apparent that the method was unable to differentiate between phytase activity and acid production. Strong positive reactions (zones of clearing around microbial colonies)observed for acid producing, anaerobic bacteria, such as Streptococcus bovis, were not corroborated by subsequent quantitative assays. Experimentation revealed that acidic solutions generated false positive results on the selected differential medium. Empirical studies undertaken to find a solution to this limitation determined the false positive results could be eliminated through a two step counterstaining treatment (cobalt chloride and ammonium molybdate/ammonium vanadate)which reprecipitates acid solubilized phytate. This report discusses the application of the developed two step counterstaining treatment for the screening of phytase producing ruminal bacteria as well as its use in phytase zymogram assays.
650	1	4	_aPHYTASE
650	1	4	_aPHYTIC ACID
650	1	4	_aPLATE SCREENING
650	1	4	_aSTAINING
650	1	4	_aZYMOGRAM
700	1	2	_aBae, H.D.
700	1	2	_aYanke, L.J.
700	1	2	_aCheng, K.-J.
700	1	2	_aSelinger, L.B.
856	4	0	_uhttps://drive.google.com/file/d/1PeNLxuVLQeNv4jxcaOiHGI8BInnYbzcr/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c47607 _d47607